Aqp5-Cre

品系全名

C57BL/6JSmo-Aqp5em1(iCre-WPRE-polyA)Smoc

目录号

NM-KI-200062

品系状态

精子冻存

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基因信息

基因名
Aqp5

品系描述

将iCre-WPRE-polyA插入到小鼠Aqp5基因起始密码子处。
应用领域:Cre工具鼠;AQP5属于水通道蛋白家族,它允许水通过渗透作用穿过质膜,在唾液腺和肺的上皮细胞等都有表达。Aqp5 -Cre与报告基因小鼠联合应用,能够标记Aqp5阳性细胞,与含floxp侧翼小鼠交配,能剔除后代含floxp之间的序列或基因。

验证数据

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图 1 Aqp5Cre/+; Rosa26tdTomato/+ 小鼠肺部tdTomato的表达情况。

Aqp5Cre/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠肺上皮细胞中检测到tdTomato的表达。

image.png

图 2 Aqp5Cre/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况。TdTomato可在多个组织中表达,包括:肺上皮细胞,唾液腺、胰腺、脑、腺胃、大肠、食道外肌层、心脏个别细胞,肾个别细胞、脾个别细胞、卵巢和子宫的个别细胞。(更多详细信息请联系我司技术顾问。)



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