Alb-CreERT2

品系全名

B6.129S-Albtm1.1(CreERT2)Smoc

目录号

NM-KI-00002

品系状态

活体

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基因信息

基因名
ALB

品系描述

将CreERT2表达框定点敲入小鼠内源Alb基因ATG位置,建立Tamoxifen诱导的Alb-Cre工具鼠。该品系已去除Neo筛选基因。该小鼠是研究肝脏基因功能与疾病的重要小鼠品系。
应用领域:肝脏特异

验证数据

alb-creERT2-1.png

Fig1 Alb-CreERT2; Rosa26-LacZ小鼠肝组织X-gal染色。未使用他莫昔芬诱导的小鼠,肝细胞未被染色;使用他莫昔芬诱导的小鼠,肝细胞成功着色,证明Alb-CreERT2小鼠可以成为实现肝脏时间特异性敲除或表达的工具鼠。

alb-creERT2-2.png

Fig2 Alb-CreERT2; Rosa26-LacZ小鼠肝组织免疫荧光检测。Alb-CreERT2; Rosa26-LacZ小鼠被他莫昔芬诱导后,LacZ(红色)表达,证明Alb-CreERT2小鼠可以成为实现肝脏时间特异性敲除或表达的工具鼠。


alb-creERT2-3.png


Fig3  Alb-CreERT2小鼠血生化检测。结果显示Alb-CreERT2小鼠与野生型小鼠相比肝功能正常。





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