hCSF1/hCSF1R(BALB/c)

品系全名

BALB/cJSmo-Csf1tm2(CSF1)Csf1rtm3(hCSF1R)Smoc

目录号

NM-HU-220016

品系状态

胚胎冻存

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品系描述

通过同源重组,将小鼠Csf1和Csf1r基因进行人源化修饰。

验证数据

image.png

Fig.1 Detection of CSF1 expression in spleen and lung by RT-PCR. 

Wild type: only one band at 299 bp with primers F1/R1(mCsf1);

Homozygous: only one band at 261 bp with primers F2/R2(hCSF1); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of CSF1R expression in spleen and lung by RT-PCR. 

Wild type: only one band at 233 bp with primers F1/R1(mCsf1r);

Homozygous: only one band at 345 bp with primers F2/R2(hCSF1R); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hCSF1 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hCSF1/hCSF1R and BALB/c mice were i.p. injected with LPS for 6h.

image.png

Fig.4 Expression characterization of hCSF1R on Monocytes/ macrophages in Bone marrow in hCSF1/hCSF1R dKI mice.



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