hIL7

Fig.1 Detection of IL7 expression in thymus by RT-PCR. 

Wild type: only one band at 177 bp with primers F1/R1(mIl7);

Homozygous: only one band at 340 bp with primers F2/R2(hIL7).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of IL7 expression in thymus homogenate by ELISA. 

Wild-type C57BL/6 mice (WT, female, 11 weeks old, n=3) and homozygous hIL7 knockin mice (HO, male, 9 weeks old, n=3) were administered poly(I:C) (100 μg/mouse) via intraperitoneal (i.p.) injection. After 6 hours, thymus homogenates were collected for IL7 measurement by ELISA. Human IL7 was detectable in the HO mice. Notably, the ELISA kit used exhibits cross-reactivity between human and mouse IL7 proteins.