hMSTN

利用同源重组，将小鼠Mstn 基因进行人源化修饰。

Fig.1 Detection of MSTN expression in mammary gland by RT-PCR. 

Wild type: only one band at 135 bp with primers F1/R1(mMstn);

Homozygous: only one band at 291 bp with primers F2/R2(hMSTN).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of hMSTN(A) and mMSTN(B) expression in serum by ELISA (female, 7-8wks, n=3). 

Abbr.  HO, homozygous; WT, wild type.

Note. The Human MSTN ELISA Kit cross-reacted with mouse MSTN and humanized MSTN.

Fig.3 In vivo efficacy of a human MSTN-targeting siRNA drug in hMSTN mice (Data from a collaborator). 

Homozygous hMSTN mice were randomly divided into two groups (female, 7 weeks old, n=5/group). Mice were administered a siRNA drug, containing the sequence from AD06569 drug. Serum MSTN levels were detected before and after dosing in hMSTN mice. Compared to the vehicle group, the siRNA treatment group demonstrated a significant decrease in serum MSTN levels. (Mean±SEM. Unpaired t-test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).