RPMI 8226-Luc


品系基本信息

验证数据

RPMI 8226-Luc 细胞系为 RPMI 8226 野生型细胞系经过转染包含 Luciferase 荧光素酶的稳转株,经检测,该细胞系中可检测到高表达 Luciferase 活性,且成瘤过程中 Luciferase 活性呈稳步上升的趋势。


一、细胞形态图

在10倍显微镜下拍摄细胞形态,如图一所示:

image.png

图一、RPMI 8226-Luc 细胞的形态图


二、细胞接种前 Luciferase 活性检测

常规在细胞接种前进行 Luciferase 活性检测,结果如图二所示:

image.png

图二、RPMI 8226-Luc 细胞接种前 Luciferase 活性检测


三、小鼠体内 Luciferase 荧光信号变化

在实验过程中持续规律性检测小鼠体内 Luciferase 荧光信号,结果如图三所示:

image.png

图三、RPMI 8226-Luc 细胞在 M-NSG 小鼠体内 Luciferase 荧光信号变化曲线图 (n=5)


四、小鼠生存曲线

在实验过程中同时也记录小鼠生存情况,结果如图四所示:

image.png

图四、小鼠生存曲线图 (n=5)


五、小鼠活体成像图

不同时间点小鼠的活体成像图,如图五所示:

image.png

图五、不同时间点小鼠活体成像图



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