hCB1(2)
品系全名
C57BL/6Smoc-Cnr1em1(hCNR1)Smoc
目录号
NM-HU-225070
品系状态
胚胎冻存
基因信息
基因名
Cnr1
品系描述
利用同源重组，将小鼠Cnr1基因进行人源化修饰。
验证数据
Fig1. Detection of CB1 expression in cerebellum by RT-PCR.
Wild type: only one band at 373 bp with primers F1/R1(mCb1);
Homozygous: only one band at 209 bp with primers F2/R2(hCB1).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig3. Detection of human CB1 expression in CA1 in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig4. Detection of human CB1 expression in CA3 in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig5. Detection of human CB1 expression in Dentate Gyrus(DG) in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig6. Detection of human CB1 expression in Cerebellum in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
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