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U-GEMMs

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Mice

hB7H3

Strain Name:C57BL/6Smoc-Cd276tm2(hB7H3)Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-HU-00107

The endogenous mouse Cd276 gene was replaced by human CD276 gene.

Mice

h4-1BB(2)/hOX40

Strain Name:C57BL/6Smoc-Tnfrsf9tm2(TNFRSF9)Tnfrsf4em1(hTNFRSF4)/Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-HU-200272

hTNFRSF9(NM-HU-190077) was crossed with hOX40(NM-HU-00041)to generate h4-1BB(2)/hOX40 mice

Mice

hCTLA-4

Strain Name:C57BL/6Smoc-Ctla4em1(hCTLA4) Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-HU-00014

The endogenous mouse Ctal4 gene was replaced by human CTLA4 gene. While hCTLA4(2)(Stock No.NM-HU-190038) mice function similarly to hCTLA4 mice,for more detailed information please contact our technical advisor.

Mice

Rag2-KO(Rag2-EGFP)

Strain Name:B6;129S-Rag2tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-KI-00070

A loxP-EGFP-PolyA-loxP-Neo-loxP expression cassette was knocked into the Rag2 gene start codon site. As a Rag2 knockout mouse model, this stain can be used in subcutaneous inoculation of liver cancer tissues and tumor cells. Tumors can esaily form and grow. The amount of T and B lymphocytes in peripheral blood of mice was extremely low tested by FACS, which was comparable to or lower than that of Nude mice, and there was a significant difference compared with wild type mice. The pathological sections of HE staining of tumor tissues showed that the tumor sections of Rag2 KO mice and Nude mice were similar. This strain has the potential to replace Nude, NOD-SCID mice as a tumor-bearing mouse model.

Mice

CAG-LSL-dCas9-SPH-Tg

Strain Name:C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain State:Repository Live | Cat. NO.:NM-TG-00025

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).

Mice

Alb-2A-DTRGFP(NMG)

Strain Name:NOD-Prkdcscid Il2rgem1Albem1(2A-DTRGFP)Smoc
Strain State:Repository Live | Cat. NO.:NM-KI-215047

2A-DTRGFP expression cassette was knocked into the Alb gene.

Mice

Aplnr-2A-DreERT2

Strain Name:C57BL/6Smoc-Aplnrem1(2A-DreERT2)Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-KI-00132

A 2A-DreERT2-SV40polyA coexpression cassette is inserted befor the stop codon of mouse Aplnr gene via homologous recombination to establish a tamoxifen inducible Aplnr-DreERT2 tool stain. This strain can be used to knockout target gene in Aplnr+ cells after tamoxifen treatment when crossed with mice which carry rox sites flanking target gene.

Mice

B2m-KO(NMG)

Strain Name:NOD.Cg-PrkdcscidIl2rgem1SmocB2mem1Smoc
Strain State:Repository Live | Cat. NO.:NM-NSG-002

This B2m knockout mouse model was obtained by knocking out the exon 2-3 region of mouse B2m gene on the background of NMG mouse.

Mice

hLAG3

Strain Name:B6.129S-Lag3tm1(hLAG3)Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-HU-00049

The endogenous mouse Lag3 gene exon1-7 was replaced by human LAG3 gene extracellular region by homologous recombination.

Mice

F9-KO

Strain Name:C57BL/6Smoc-F9em1Smoc
Strain State:Embryo cryopreservation | Cat. NO.:NM-KO-18046

The F9 gene is located on the X chromosome. In this F9 knockout mouse model, gRNAs were designed targeting Exon8 of F9 gene via CRISPR gene editing technology. Loss of F9 caused coagulopathy in mice. Mice that are homozygous for F9 knockout are viable, fertile and normal in size. Normal fighting in the cage may cause bleeding or even death due to massive internal hemorrhaging. After the tail cutting, wounds must be cauterized to prevent homozygous knockout mice from blood loss and death. This strain is a powerful model for studying coagulopathy, gene therapy methods and function of factor IX mutations. F9-KO mice (Stock No.NM-KO-18046)carry a knockout allele derived from the targeted deletion of exon 8. While F9-KO(2) mice (Stock No.NM-KO-200607) carrying the exon 1-8 deletion.

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