Gene-editing proposal
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Strain Name: C57BL/6J-Il2em1(Venus-AkaLuc)Smoc Mutation Type: Knock-in Research Application: Gene tracing
Using CRISPR/Cas9 gene editing technology, an IRES-Venus AkaLuc cassette was inserted into the IL2 gene stop codon site.
Strain Name: C57BL/6-Igs2em1(CAG-RSR-EYFP)Smoc Mutation Type: Knock-in Research Application: Cell tracing
A CAG-ROX-STOP-ROX-EYFP-V5tag-polyA conditional expression cassette was knocked into the Igs2 locus (Hipp11 or H11; an intergenic region on chromosome 11 [cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci] reported to support high-level global gene expression in conjunction with the CAG (CAGGS) promoter and a higher rate of mitotic/interchromosomal recombination compared to Gt(ROSA)26Sor) by CRISPR/Cas9 gene editing technology. This strain of mice can be used to mate with mice expressing the Dre recombinase. After exposure to Dre recombinase, expression of ellow fluorescent protein and V5 tag is observed for cell tracking.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-EYFP)Smoc Mutation Type: Knock-in Research Application: Cell tracing
A SA-2xpolyA-CAG-Loxp-Stop-Loxp-EYFP-polyA cassette was knocked into the mouse ROSA26 gene locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Cre recombinase so that yellow fluorescent protein could be expressed after exposure to Cre recombinase.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-RSR-mTagBFP2)Smoc Mutation Type: Knock-in Research Application: Cell tracing
A SA-2xpolyA-CAG-ROX-stop-ROX-mTagBFP2-polyA cassette was knocked into the mouse ROSA26 locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Dre recombinase so that blue fluorescent protein could be expressed after exposure to Dre recombinase.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-EZH2-IRES-luciferase-2A-tdTomato)Smoc Mutation Type: Knock-in Research Application: Tumor research; drug screening
A SA-2xpolyA-CAG-Loxp-Stop-Loxp-human EZH2 cDNA-IRES-luciferase-2A-tdTomato-wpre-polyA cassette was knocked into the mouse Rosa26 locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Cre recombinase so that human EZH2 could be expressed after exposure to Cre recombinase.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-c-MET-IRES-luciferase-2A-tdTomato)Smoc Mutation Type: Knock-in Research Application: Tumor research; drug screening
A SA-2xpolyA-CAG-Loxp-Stop-Loxp-human cMET-IRES-luciferase-2A-tdTomato-wpre-polyA cassette was knocked into the mouse Rosa26 locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Cre recombinase so that human C-MET could be expressed after exposure to Cre recombinase.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-Akaluc-2A-tdTomato-V5)Smoc Mutation Type: Knock-in Research Application: Cell tracing
A CAG-loxP STOP-loxP-Akaluc-2A-tdTomato-V5-WPRE-polyA cassette was knocked into the mouse ROSA26 locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Cre recombinase so that Akaluc luciferase and tdTomato fluorescent protein could be expressed for cell tracing.
Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-NRF2-IRES-luciferase-2A-tdTomato)Smoc Mutation Type: Knock-in Research Application: Tumor research; drug screening
A SA-2xpolyA-CAG-LSL-human NRF2-IRES-luciferase-2A-tdTomato-wpre-polyA cassette was knocked into the mouse Rosa26 locus by CRISPR/Cas9 gene editing technology. This strain of mice can be mated with mice expressing Cre recombinase to express human NRF2 and a reporter gene in certain cells or tissues that expressing Cre recombinase.
Strain Name: C57BL/6J-Piezo1tm2(CreERT2)Smoc Mutation Type: Knock-in Research Application: Mechanically sensitive ion channel Cre tool mouse
This tamoxifen-inducing Piezo1-CreERT2 tool mouse model was established by inserting a 2A-CreERT2 co-expression cassette into the Piezo1 gene stop codon site by CRISPR/Cas9 gene editing technology.
Strain Name: C57BL/6-Igs2em1(pThy1-PSEN1-IRES-tdTomato-WPRE-pA)Smoc Mutation Type: Knock-in Research Application: Alzheimer's research
A Thy1 promoter-PSEN1-IRES-tdTomato-WPRE-polyA cassette was knocked into the Igs2 locus (Hipp11 or H11; an intergenic region on chromosome 11 [cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci] reported to support high-level global gene expression in conjunction with the CAG (CAGGS) promoter and a higher rate of mitotic/interchromosomal recombination compared to Gt(ROSA)26Sor) by CRISPR/Cas9 gene editing technology.