Generating knock-in mouse models can simulate the human genetic mechanism, explore the pathogenic mechanism, and simplify the process of drug development.
Simulate human genetic mechanism, and explore pathogenic mechanism
Gene expression tracking
Genetic lineage tracing defines cell origin
Create humanized mouse model to accelerate drug research and development
Introduce the tools of molecular genetics into mouse models
Knock-in models include:
It usually takes 6-9 months to generate a conditional gene knock-in mouse model by CRISPR gene editing technology.
It usually takes 9-12 months to generate a conditional gene knock-in mouse model by ES cell targeting technology.
Shanghai Model Organisms Center (SMOC) has more than 900 research-ready GEM models and one of them may contain the strain that you are interested in. Click here to search the SMOC Research-Ready Models Repository.
Alternatively, you may contact our technical consultants to design and customize your gene knock-in mouse model.
Conventional gene knock-in
Co-expression of exogenous genes:
Replacement of murine endogenous genes with exogenous genes (i.e. knock-in and knockout simultaneously):
Introduction of point mutations (human pathogenic point mutation candidates) into the corresponding positions of murine homologous genes.
Conditional point mutation
Tissue-specific point mutations can be achieved by combining point mutations (human pathogenic point mutation candidates) to the Cre-LoxP system and introducing them into the corresponding positions of mouse homologous genes.
Conditional point mutations can be achieved by inserting two loxp sites and the exon with mutations.
The mouse endogenous gene was replaced with a human homologous gene to generate a humanized genetically engineered mouse model for disease, immunity, physiological research, and antibody drug screening and drug efficacy evaluation.
Direct insertion of human cDNA：