F9-KO

Nomenclature

C57BL/6Smoc-F9em1Smoc

Cat. NO.

NM-KO-18046

Strain State

Embryo cryopreservation

Export PDF

Gene Summary

Gene Symbol
F9

Model Description

The F9 gene is located on the X chromosome. In this F9 knockout mouse model, gRNAs were designed targeting Exon8 of F9 gene via CRISPR gene editing technology. Loss of F9 caused coagulopathy in mice. Mice that are homozygous for F9 knockout are viable, fertile and normal in size. Normal fighting in the cage may cause bleeding or even death due to massive internal hemorrhaging. After the tail cutting, wounds must be cauterized to prevent homozygous knockout mice from blood loss and death. This strain is a powerful model for studying coagulopathy, gene therapy methods and function of factor IX mutations. F9-KO mice (Stock No.NM-KO-18046)carry a knockout allele derived from the targeted deletion of exon 8. While F9-KO(2) mice (Stock No.NM-KO-200607) carrying the exon 1-8 deletion.
Research Application:factor IX (F9) function and gene therapy

Disease Connection

Hemophilia B

Validation Data

f9-7.png

Fig7 Generation of F9 mutant mouse strain.

f9-8.png

Fig8 Expression of F9 mRNA in the hepatic tissue of WT and KO mouse strains.

f9-9.png

Fig9 Concentration of FIX protein in the plasma of WT and KO mouse strains.

Data in c and d were acquired from 8 mice per group, and are shown as the mean ± the s.d.

Twotailed unpaired Student’s t tests were used to determine the P value.**P < 0.01



You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Learn more