Dysgenesis model


Prss37 gene knockout mouse infertility model

SMOC has independently developed the Prss37 gene knockout mouse model (Prss37 KO, Model No.: NM -KO-00016) by employing ES cell targeting. It is a homozygous male infertility model.

image.png


The following phenotype is demonstrated:

  • The Prss37 KO infertile mice exhibit normal spermatogenesis, sperm morphology and motility. In addition, A23187-induced sperm acrosome reaction and acrosomal enzyme activity in the infertile mice are comparable to those in wild-type mice.

  • The in vivo fertilization rate of Prss37 KO infertile mice is severely impaired as there are defects in sperm migration from the uterus to the oviduct.

  • The sperms from Prss37 KO infertile mice are unable to penetrate the zona pellucida and fuse with the ovum.

  • The mature Adam3 is absent from Prss37 KO infertile mice.


image.png

Fig 1. Prss37 gene knockout mouse generation strategy and expression validation

 

 image.png

Fig 2. The sperms from Prss37 gene knockout mice are unable to penetrate the zona pellucida and fuse with the ovum


Reference
Shen C, Kuang Y, Liu J, et al. Prss37 is required for male fertility in the mouse. Biol Reprod. 2013 May 16;88(5):123.



Popular articles

Workshop:Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

Learn more
Shanghai Model Organisms Jinshan R&D Base officially put into operation

After the base is put into operation, SMOC’s capability to provide genetically modified rat/mouse models and technical services including gene function research and drug development will be greatly enhanced.

Learn more