hTLR8

Nomenclature

C57BL/6Smoc-Tlr8tm2(TLR8)Smoc

Cat. NO.

NM-HU-190044

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
TLR8

Model Description

The endogenous mouse Tlr8 gene was replaced by human TLR8 gene.
Research Application:Immunotherapy,cancer research,drug screening

Validation Data

image.png

Fig1. Detection of TLR8 expression in spleen, lung and thymus by RT-PCR. Wild type: only one band at 318 bp with primers F1/R1(mTlr8);Homoygous: only one band at 366 bp with primers F2/R2(hTLR8); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. The representative picture of hepatomegaly(top), splenomegaly (bottom) in aged hTLR8 mice.

image.png


Fig 3. Histology of (A) spleen and (B) liver in aged hTLR8 mice. A prominent portal inflammation with fibrosis and bile duct hyperplasia in liver (A); Decreased cellularity in periarteriolar lymphoid sheath and the increase of histiocytes in the red pulp of spleen (B). 

image.png

Fig 4. The expression pattern of hTLR8 in myeloid cells (A, B); and cytokine analysis in serum of aged hTLR8 mice (C).

image.png

Fig 5. The dynamic change of (A) myeloid and (B) lymphocyte lineage in the spleen of hTLR8 mice; and (C) in vitro stimulation of splenocytes from wild type (WT) C57BL/6 and hTLR8 mice with hTLR8 agonist ORN-06

image.png

Fig 6. MC-38 tumor growth curve and tumor weight of individual mice from the 4 groups. One way ANOVA *, **, and *** refer to p<0.05, p<0.01, and p<0.001, respectively

image.png

Fig 7. Serum level of cytokine analysis by MSD and intracellular TNF-a staining by FACS in splenocytes.

image.png

Fig 8. Tumor infiltrated immune component analysis.

image.png

Fig 9. Pro-inflammatory cytokines in the tumor cell lysate. *Validation data are provided by Crownbio.


You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Learn more