hIL6/hIL6R

Nomenclature

C57BL/6Smoc-Il6tm1(hIL6)Il6raem1(hIL6R)/Smoc

Cat. NO.

NM-HU-190025

Strain State

Repository Live

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Gene Summary

Gene Symbol
IL6

Synonyms

Il; Il-6

NCBI ID

16193

MGI ID

96559

Ensembl ID

ENSMUSG00000025746

Pubmed

IL6

Human Ortholog

IL6

Gene Symbol
IL6Ra

Synonyms

Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha

NCBI ID

16194

MGI ID

105304

Ensembl ID

ENSMUSG00000027947

Pubmed

IL6Ra

Human Ortholog

IL6RA

Model Description

This IL6 and IL6R double knockin strain is established by crossing IL6-HU and IL6R-HU mice together. These double double knockin mouse models can be useful for evalueting the efficacy of potential immunotherapy drug combinations.
Research Application：Immune-related

Validation Data

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Fig1 The expression of human IL6 in liver tissue of humanized IL6/IL6R mice was measured by qPCR. The results showed that the active expression of humanized IL6 can be detected in liver tissue of homozygous humanized IL6/IL6R mice after LPS Activation. 

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Fig2 Expression of human IL6 in the serum of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6 can be detected in the serum collected from homozygous mice after LPS Activation. 

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Fig3 Expression of human IL6R in the serum, liver and pancreas of humanized IL6/IL6R homozygous mice is detected by Elisa. The results showed that the expression of human IL6R can be detected in the serum, liver and pancreas collected from homozygous mice. 

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Fig4. Detection of hIL-6R expression in MM.1S cells.

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Fig5. Detection of hIL-6R expression in T cells in hIL-6/hIL-6R dKI mice.

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Fig6. Detection of hIL-6R expression in Myeloid cells, T cells and activated B cells in hIL-6/hIL-6R dKI mice. Note.  Splenocytes from C57BL/6 and homozygous IL-6/hIL-6R mice were stimulated with LPS in vitro. 

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Fig7. Detection of hIL-6R expression in brainstem in hIL-6/hIL-6R dKI mice by IHC.

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Fig8. Detection of hIL-6R expression in spleen in hIL-6 /hIL-6R dKI mice by IHC.

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Fig9. Detection of hIL-6R expression in lung and thymus in hIL-6 /hIL-6R dKI mice by IHC.

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