Cd8a-(V5-IRES-Akaluc-2A-EGFP)


Strain Information

Strain Name

C57BL/6J-Cd8aem1(V5-IRES-Akaluc-2A-EGFP)Smoc

Cat. NO.

NM-KI-18030

Gene Symbol

Cd8a

Gene Synonyms

Ly-2, Ly-35, Ly-B, Lyt-2

Human Ortholog

CD8A

Ensembl ID

MGI ID

NCBI ID


Description

Using CRISPR gene editing technology, a V5-IRES-Akaluc-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knockin model with V5, Akaluc luciferase and EGFP fluorescent protein. Akaluc is a novel luciferase that produces up to 1,000 times as bright as light emitted by existing systems by feeding or injecting a synthetic luciferin compound called AkaLumine-HCl, which is better able to penetrate the blood-brain barrier than d-luciferin is. Click here for details.


Research application

gene tracing

Status

Availability

Repository Live


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Milestones

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