Strain Information

Strain Name


Cat. NO.


Gene Symbol


Gene Synonyms

1010001H21Rik, 4922501H04Rik, LC3, LC3a

Human Ortholog


Ensembl ID




The CAG promoter-loxp-stop-loxp-mCherry-EGFP-Map1lc3a-WPRE-polyA expression cassette was inserted into the Rosa26 gene locus by homologous recombination using CRISPR/Cas9 technology. Map1lc3a, also known as LC3, is a widely expressed autophagic vesicle-specific marker. There was no abnormality in heterozygous mice. The presence of the loxp-stop-loxp expression cassette prevented the transcription of the downstream target gene LC3. After mating with Cre mice,  LC3 gene is driven by the CAG promoter to express mCherry and EGFP in a pH-dependent manner in phagocytic cells after ischemic injury in the progeny double positive mice. The two co-expressed fluorescent signals vary depending on the acidic environment of the autophagic vesicles within the cell. mCherry is stable in an acidic environment (pKa 4.5), and quenching occurs in the acidic environment (pKa 5.9) of EGFP in lysosomes. In the autophagy vesicles with higher pH, the fluorescence of GFP and mCherry superimposed on yellow fluorescence; while in the lower pH lysosome, EGFP was quenched and only red fluorescence signal was detected. It can be used to label and track LC3 and study the origin, progression and disappearance of autophagy in various tissues after ischemic injury.

Research application



Repository Live

Related Articles

Branding Strategy Upgrade

The Company will be embarking on a brand upgrade project from April 2018 to better serve our clients. "南模生物 (Shanghai Model Organisms)" will be adopted as the Company's Chinese brand name, together with a new corporate logo. This will coincide with the launch of a new corporate website: www.modelorg.com.


Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Contact us Email Gene-editing proposal Gene-editing proposal Back to top Back to top