M-NSG Severely Immunodeficient Mouse

Our own-developed M-NSG mice used the CRISPR/Cas9 technique to knock out both Prkdc and Il2rg genes from NOD mice to obtain severely immunodeficient mice. These mice lack mature T, B, and NK cells and are an important carrier for humanized mice, xenografts, and immune reconstitution. They also have significant values for the study on human hematopoietic stem cells, tumorigenesis and tumor treatment, immunodeficiency diseases, and immune mechanisms in the body.

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M-NSG Mouse(NOD-PrkdcscidIl2rgem1/Smoc)

Developed by Shanghai Model Organisms, the proprietary Il2rg knockout mice have a NOD SCID background

strain information

Strain Name  NOD-PrkdcscidIl2rgem1/Smoc

Cat. NO. NM-NSG-001

Genotype Male: Prkdc-/- , IL2rg-/Y ; Female:Prkdc-/- , IL2rg-/-

Description

Prkdc and Il2rg genes were both knocked-out in NOD mice. These mice lack mature T cells, B cells as well as NK cells.


Characteristics

Lacking mature T, B and NK cells

Low immune rejection against human cells and tissues

Good tumorigenicity so that a small number of cells can form tumors.

Significant improvement in the survival of transplanted human cells and tissues

Suitable for the transplantation of human hematopoietic stem cells and the preparation of humanized mouse models

Suitable as the carrier mice for the transplantation of heterologous cells and tissues


FACS detection of T, B and NK cells collected from M-NSG mice

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Figure 1. Complete deletion of T, B and NK cells in the spleen of M-NSG mice.

The splenocytes of C57BL/6, NOD-scid and M-NSG mice were collect to analyze their compositions of T, B and NK cells by FACS.

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Figure 2. Complete deletion of T, B and NK cells in the blood of M-NSG mice.

The peripheral blood samples of C57BL/6, NOD-scid and M-NSG mice were collect to analyze their compositions of T, B and NK cells by FACS.

A CDX tumor model of M-NSG mice

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Figure 3. The establishment of tumor models using A549 lung cancer cells is more effective in M-NSG mice.

3x106 of A549 cells were inoculated subcutaneously into C57BL/6, Balb/c Nude, NOD-scid and M-NSG mice, respectively, and the tumor volumes were measured at different time points.

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Figure 4. The establishment of tumor models using Raji lymphoma cells is more effective in M-NSG mice.

1x106 of Raji cells were inoculated subcutaneously into C57BL/6, Balb/c Nude, NOD-scid and M-NSG mice, respectively, and the tumor volumes were measured at different time points.

A CDX model has been successfully established in M-NSG mice:

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Efficacy experiment of CDX model of M-NSG mice图片1.png

Figure 5. A549 cells mixed up with h-PBMC at ration 3 for future study.


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Figure 6. Calu-6 cells mixed up with h-PBMC at ration 3 and 4 for future study.

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