Ascl1-CreERT2

Nomenclature

C57BL/6Smoc-Ascl1em1(CreERT2-Wpre-pA)Smoc

Cat. NO.

NM-KI-200207

Strain State

Embryo cryopreservation

Export PDF

Gene Summary

Gene Symbol
Ascl1

Model Description

A CreERT2-Wpre-pA expression cassette was knocked into the Ascl1 gene start codon site.
Research Application:When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre after tamoxifen treatment. These mice may be useful in studying the role of Ascl1 positive neural progenitor cells in the neuronal turnover and neuronal replacement.

Validation Data

NM-KI-200207;Ascl1-CreERT2-1.png

Fig. 1 CreERT2-mediated recombination in the hippocampus of Ascl1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the dentate gyrus granule cell layer and molecular layer of Ascl1 CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. Some leakiness were detected in the molecular layer prior to tamoxifen exposure. 

NM-KI-200207;Ascl1-CreERT2-2.png

Fig. 2 CreERT2-mediated recombination in the lateral ventricles of Ascl1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the subventricular zone of Ascl1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200207;Ascl1-CreERT2-3.png

Fig. 3 Detection of tdTomato(red) in various tissues of Ascl1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the hippocampus and lateral ventricles after tamoxifen treatment. Besides, tamoxifen-dependent cre recombinase expression can be also detected in some cells derived from thymus and the submucosa of the stomach and small intestine, except for submandibular gland, retina, lung, brown fat, kidney, muscle and spleen. (For more detailed information please contact our technical advisor.)


You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Learn more