Foxp3-IRES-tdTomato-2A-Cre

Fig. 1 Cre-mediated recombination in the spleen and thymus of Foxp3^Cre/+; Rosa26^Zsgreen/+ mouse. Zsgreen(green) expression can be detected in the spleen and thymus of Foxp3^Cre/+; Rosa26^Zsgreen/+ mouse, while tdTomato(red) expression can not be detected.

Fig. 2 Flow cytometry analysis of zsGreen and tdTomato expression in the Foxp3⁺ cell derived from the thymus of mice. ZsGreen⁺ cells accounted for 37.56% of Foxp3⁺ cells in the thymus of Foxp3^Cre/+; Rosa26^Zsgreen/+ mouse, while tdTomato+ cells accounted for 1.27%.

Fig. 3 Flow cytometry analysis of zsGreen and tdTomato expression in the Foxp3⁺ cell derived from the spleen of mice. Zsgreen⁺ cells accounted for 84.74% of Foxp3⁺ cells in the spleen of Foxp3^Cre/+; Rosa26^Zsgreen/+ mouse, while tdTomato+ cells accounted for 0.46%.

Fig. ４ ZsGreen was also expressed in the mesenteric lymph nodes, pancreas and liver. There was a small amount of expression in the brain, pituitary gland, retina, lung, stomach, kidney, submandibular gland, brown fat, ovary and skin, and no expression in the heart, colon and muscle.（For more information please contact: 400-728-0660.）