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Col2a1-(2A-CreERT2) Mouse

Strain Name: C57BL/6J-Col2a1em1(V5-Avi-IRES-Akaluc-2A-tdTomato)Smoc Mutation Type: Knock-in Research Application: Cre recombinase tool

Using the CRISPR gene editing technique, a 2A-CreERT2 expression cassette was knocked into the stop codon of Col2a1 gene to establish a Col2a1-CreERT2 recombinase tool mouse.

Cd8a-(V5-IRES-Akaluc-2A-EGFP) Mouse

Strain Name: C57BL/6J-Cd8aem1(V5-IRES-Akaluc-2A-EGFP)Smoc Mutation Type: Knock-in Research Application: gene tracing

Using CRISPR gene editing technology, a V5-IRES-Akaluc-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knockin model with V5, Akaluc luciferase and EGFP fluorescent protein. Akaluc is a novel luciferase that produces up to 1,000 times as bright as light emitted by existing systems by feeding or injecting a synthetic luciferin compound called AkaLumine-HCl, which is better able to penetrate the blood-brain barrier than d-luciferin is.

R26-(CAG-LSL-Avi-dCas9-IRES-EGFP-WPRE-pA) Mouse

Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-Avi tag-dCas9-IRES-EGFP-WPRE-pA)Smoc Mutation Type: Knock-in Research Application:

A CAG promoter-loxp-stop-loxp-Avi tag-dCas9-IRES-EGFP-WPRE-polyA expression cassette was inserted at the Rosa26 locus site using CRISPR gene editing technology.

R26-(Liver-CYP3A4) Mouse

Strain Name: FVB-Gt(ROSA)26Sorem1(Liver-CYP3A4)Smoc Mutation Type: Knock-in Research Application: metabolism

This knock-in model was generated by inserting the human CYP3A4 cDNA driven by the liver-specific APOE promoter into the mouse Rosa26 site, which can be used to obtain liver-expressed CYP3A4 humanized model after mating with the Cyp3a13 gene and other Cyp3a family gene knockout mice. This model can be used with NM-KI-18033 mice which has gut-specific expression of human CYP3A4 to compare the contribution of intestinal versus hepatic metabolism to the biotransformation of a test article.

R26-(Gut-CYP3A4) Mouse

Strain Name: FVB-Gt(ROSA)26Sorem1(Gut-CYP3A4-IRES-tdTomato)Smoc Mutation Type: Knock-in Research Application: metabolism

Intestinal expression of the CYP3A enzyme in the human body can cause significant intestinal metabolism of the compound, resulting in impaired drug absorption. This knock-in model was generated by inserting the human CYP3A4 cDNA driven by the Villin1 promoter together with the IRES-tdTomato reporter gene into mosue Rosa26 site, which can be crossed with the Cyp3a13 gene knockout and other Cyp3a family genes knockout mice to obtain intestinal-expressed CYP3A4 humanized mouse model in order to determine the contribution of intestinal metabolism to the absorption and distribution of test article.

Foxp3-(V5-Avi tag-IRES-Akaluc-2A-tdTomato) Mouse

Strain Name: C57BL/6J-Foxp3em1(V5-Avi-IRES-Akaluc-2A-tdTomato)Smoc Mutation Type: Knock-in Research Application: gene tracing

Using CRISPR gene editing technology, a V5-Avi-IRES-Akaluc-2A-tdTomato expression cassette was inserted before the stop codon TGA of mouse Foxp3 gene to establish a multi-labeled knockin model with V5, Avi, Akaluc luciferase and tdTomato fluorescent protein. Akaluc is a novel luciferase that produces up to 1,000 times as bright as light emitted by existing systems by feeding or injecting a synthetic luciferin compound called AkaLumine-HCl, which is better able to penetrate the blood-brain barrier than d-luciferin is.

R26-(CAG-LSL-V5-TERT-IRES-Luciferase-2A-EGFP-WPRE-pA) Mouse

Strain Name: C57BL/6J-Gt(ROSA)26Sorem1(CAG-LSL-V5-TERT-IRES-Luciferase-2A-EGFP-WPRE-pA)Smoc Mutation Type: Knock-in Research Application: Telomerase

A CAG promoter-loxp-stop-loxp-V5-TERT-IRES-Luciferase-2A-EGFP-WPRE-polyA expression cassette was inserted at the Rosa26 locus site using CRISPR gene editing technology.

H2-Ab1/H2-Ea-ps-KO Mouse

Strain Name: C57BL/6J-H2-Ab1em1Smoc H2-Ea-ps em1 Smoc Mutation Type: Knockout Research Application: immune system

All sequences from the upstream of mouse H2-Ab1 gene exon 2 to the upstream of the H2-Ea-ps gene exon 1 were knocked out by CRISPR gene editing technology to obtain H2-Ab1 and H2-Ea-ps double knockout mouse models. These MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins.

Fcgr1-KO Mouse

Strain Name: C57BL/6J-Fcgr1em1Smoc Mutation Type: Knockout Research Application: immunodeficiency, immune system

The Fcgr1 knockout mouse model was obtained by knocking out all coding regions of mouse Fcgr1 gene by CRISPR gene editing technology.

Ildr2-KO Mouse

Strain Name: C57BL/6J-Ildr2em1Smoc Mutation Type: Knockout Research Application: Metabolism, endocrine

The Ildr2 knockout mouse model was obtained by knocking out the exon 2-3 region of mouse Ildr2 gene by CRISPR gene editing technology.

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